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Nitrocellulose-SPMA
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Ultrafiltration
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Dialysis/lyophilization
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75 % methanol precipitation
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|---|
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Spot number mean ± SD (inter-CV)
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197 ± 15 (7.6 %)
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196 ± 15 (7.6 %)
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192 ± 17 (8.8 %)
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199 ± 17 (8.4 %)
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|
%Yield mean ± SD (inter-CV)
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41.9 ± 2.6 (6.4 %)
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42.9 ± 0.6 (1.5 %)
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62.1 ± 8.4 (13.6 %)
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55.6 ± 7.0 (12.6 %)
|
|
Need of specialized/high-cost instrument
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No
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No
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Yes (lyophilizer)
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Yes (superspeed centifuge)
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Working time (min)
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15
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35
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>1440
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65
|
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Step of procedure
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3
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3
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3
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5
|
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Unit cost (US$/5-ml urine)
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0.3
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6.3
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0.3
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1.8
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-
Abbreviation: inter-CV inter-assay coefficient of variation
