Screening differentially expressed proteins from co-cultured hematopoietic cells and bone marrow-derived stromal cells by quantitative proteomics (SILAC) method

Table 1 Regulated proteins of KG1a after co-culture with HS5 in over-represented KEGG pathways: NOD-like receptor signaling pathway and platelet activation

KEGG pathway	FDR p-value	Protein accessions	Gene names	Protein descriptions	Average ratio H/M
NOD-like receptor signalling pathway	1.67E−4	P07858	CTSB	Cathepsin B	3.66
		Q5XLA6	CARD17	Caspase recruitment domain-containing protein 17	2.91
		P21796	VDAC1	Voltage-dependent anion-selective channel protein 1	2.51
		P45880	VDAC2	Voltage-dependent anion-selective channel protein 2	2.46
		Q96PP8	GBP5	Guanylate-binding protein 5	2.38
		P43490	NAMPT	Nicotinamide phosphoribosyltransferase	1.89
		Q96PP9	GBP4	Guanylate-binding protein 4	1.73
		P32455	GBP1	Guanylate-binding protein 1	1.60
Platelet activation	1.5E−3	P43405	SYK	Tyrosine-protein kinase SYK	0.61
		Q01970	PLCB3	1-Phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3	0.58
		Q9BV40	VAMP8	Vesicle-associated membrane protein 8	0.56
		Q7LDG7	RASGRP2	Calcium and DAG-regulated guanine nucleotide exchange factor I	0.56
		P08754	GNAI3	Guanine nucleotide-binding protein G(k) subunit alpha	0.54
		O00161	SNAP23	Synaptosomal-associated protein 23	0.49
		P07948	LYN	Tyrosine-protein kinase Lyn	0.30

Columns list protein UniProt accession numbers, gene names, protein descriptions, and SILAC average ratios from two biological replicates

ISSN: 1559-0275