Time dependent effect of cold ischemia on the phosphoproteome and protein kinase activity in fresh-frozen colorectal cancer tissue obtained from patients

Table 2 Overview of the number of detected phosphopeptides by MS-based phosphoproteomics in colorectal cancer tissue samples, including the number of phosphopeptides that significantly change with CIT (p < 0.01)

Number of patients	3
Number of samples	12
Total number of peptides	10,488
Average number of peptides per sample	6,044			FC > 2			FC > 3
T0 versus T60	28 (0.46%)	Up	18	15 (0.25%)	Up	10	4 (0.07%)	Up	3
		Down	10		Down	5		Down	1
T0 versus T120	48 (0.79%)	Up	23	31 (0.51%)	Up	15	16 (0.26%)	Up	7
		Down	25		Down	16		Down	9
T0 versus T180	52 (0.86%)	Up	31	23 (0.38%)	Up	15	13 (0.22%)	Up	7
		Down	21		Down	8		Down	6
Overlap between time points	2 (0.03%)	Up	1	1 (0.02%)	Up	0	1 (0.02%)	Up	0
		Down	1		Down	1		Down	1
Number of peptides at T = 0*	2715
Unstable peptides compared to T = 0	90 (3.3%)

FC, intensity fold change of Tx versus T0; *identified in all three T, 0 samples

ISSN: 1559-0275